A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Clone Dmel\bs36b08

General Information
Symbol Dmel\bs36b08 Species D. melanogaster
Name bs36b08 FlyBase ID FBcl0056131
Feature type cDNA_clone Created / Updated 2005-11-01/2006-08-19
Computed gene(s) Dmel\CG9975
Collection Status N/A
Known Problems none
Library BS
Strain y w [67cl]
Tissue Source adult stage testis & seminal vesicle & ejaculatory duct
Vector pBluescript_SK(-)  
hide Sequence Data of the Insert
Full Length Sequence
Total bases 629
GenBank
Full Length sequence data GCACGAGCGCAAACCGACTCAAGCGTCGATATAGTGACCCCCCCAAAAAA
AAAAATCAAAAAATCCAAACAAAACAACATTGGAAAACTGGGGCCTCGGC
GTGGAGAGGGATCAGATCATTTAAGAAAACCTGGTCTTCTAAACAAAAAA
AAATTATAAATGTATAACTGCATTTAAGATGAAACAGCTCGAAAAAATGC
GCGAGCAACGAAAACTGGCGATCCTGATCCTTGAAGGATTGCTTTTCGAT
TGCTCGACGTAGGCGCAACGCAAACACTAGGGAATTAGTTTTAGATAATC
ACACTTACAATCTCTCTAGCCCTCGATATCCTTGTTTTTATACGCATACC
GTTATTTAGTACAGGCAATTCAGGTGGAATAGGCTCTGGAAAATCCACCT
GTTGCCTGAAACGCTGGCCAAGACAATGCTATTTCGATCAGGGCCAGCGT
TTGGTGTTATAAAAAGAATGAACTTGGTTCGCGTTTCGCTTCTTTTCGGC
CCTCACAGTATAGCAAAATTAAGTTTCCAACGATTTTTTAAACGTTCGAC
ACGTACTTGAAATGCAACAAATTCAGCAAGATTGAAAATCGAAAGTTGCA
AAATTTCCACAAAAAAAAAAAAAAAAAAA
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Comments
  • A testis Drosophila cDNA library was constructed by Justen Andrews in Brian Oliver's laboratory at the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) which is part of the National Institutes of Health (NIH) at Bethesda, MD, USA. Poly (A) + RNA was prepared from dissected testes from 1-5 day old y w [67cl] Drosophila melanogaster flies, after removal of the paragonia and inclusion of the ejaculatory ducts and seminal vesicles. The size fractionated cDNA (1-6kb) was directionally cloned in the Stratagene Uni-Zap XR vector using EcoRI and XhoI restriction sites. pBluescript SK (-) is produced after excision. The host strain is E.coli SOLR.
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Reported As
Symbol Synonym
BS
 
hide Library References
Research paper
Andrews et al., 2000, Genome Res. 10(12): 2030--2043
Gene discovery using computational and microarray analysis of transcription in the Drosophila melanogaster testis. [FBrf0132348]
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Linkouts
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Reported As
Symbol Synonym
Secondary FlyBase IDs
hide References ( 0 )
Research paper
Andrews et al., 2000, Genome Res. 10(12): 2030--2043
Gene discovery using computational and microarray analysis of transcription in the Drosophila melanogaster testis. [FBrf0132348]