Allele Disc\T:Disc\RFP^DsRed2

General Information
Symbol	Disc\T:Disc\RFP^DsRed2	Species	D. sp.
Name		FlyBase ID	FBal0190214
Feature type	allele	Created / Updated	2006-08-22/2008-02-15
Associated gene	Disc\T:Disc\RFP

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: V105A. Amino acid replacement: I161T. Amino acid replacement: S197A. Amino acid replacement: K5E. Amino acid replacement: K9T. Amino acid replacement: R2A. Disc\RFP with the following sequence changes: 1. a series of silent base-pair changes corresponding to human codon-usage preferences for high expression in mammalian cells. 2. six amino acid substitutions which result in the more rapid appearance of red fluorescence in transfected cell lines, and an increase the emission intensity.
Assay mode
Tags	mir-7^{Scer\UAS.T:Disc\RFP-DsRed2} (Lai et al., 2005) mir-4^{Scer\UAS.T:Disc\RFP-DsRed2} (Lai et al., 2005, Lai et al., 2005) mir-11^{Scer\UAS.T:Disc\RFP-DsRed2} (Lai et al., 2005) mir-iab-4-5p^{Scer\UAS.T:Disc\RFP-DsRed2} (Ronshaugen et al., 2005) mir-2a-2^{mir2.Scer\UAS.T:Disc\RFP-DsRed2} (Lai et al., 2005, Lai et al., 2005) mir-286^{Scer\UAS.T:Disc\RFP-DsRed2} (Lai et al., 2005, Lai et al., 2005) mir-iab-4-3p^{Scer\UAS.T:Disc\RFP-DsRed2} (Ronshaugen et al., 2005) mir-5^{Scer\UAS.T:Disc\RFP-DsRed2} (Lai et al., 2005, Lai et al., 2005) mir-2a-1^{mir2.Scer\UAS.T:Disc\RFP-DsRed2} (Lai et al., 2005, Lai et al., 2005) mir-2b-2^{mir2.Scer\UAS.T:Disc\RFP-DsRed2} (Lai et al., 2005, Lai et al., 2005) mir-79^{Scer\UAS.T:Disc\RFP-DsRed2} (Lai et al., 2005) TwdlD^{T:Disc\RFP-DsRed2} (Guan et al., 2006) TwdlH^{T:Disc\RFP-DsRed2} (Guan et al., 2006) TwdlF^{T:Disc\RFP-DsRed2} (Guan et al., 2006) mir-1003^{Scer\UAS.T:Disc\RFP-DsRed2} (Okamura et al., 2007) mir-1003^{Scer\UAS.T:Disc\RFP-DsRed2,T:Hsap\MYC} (Okamura et al., 2007) mir-1003^{mut.Scer\UAS.T:Disc\RFP-DsRed2,T:Hsap\MYC} (Okamura et al., 2007) mir-1003^{miRNA.Scer\UAS.T:Disc\RFP-DsRed2} (Okamura et al., 2007) mir-1008^{Scer\UAS.T:Disc\RFP-DsRed2} (Okamura et al., 2007) mir-1008^{Scer\UAS.T:Disc\RFP-DsRed2,T:Hsap\MYC} (Okamura et al., 2007) mir-1008^{mut.Scer\UAS.T:Disc\RFP-DsRed2,T:Hsap\MYC} (Okamura et al., 2007) mir-1010^{Scer\UAS.T:Disc\RFP-DsRed2} (Okamura et al., 2007) mir-1004^{Scer\UAS.T:Disc\RFP-DsRed2} (Okamura et al., 2007) mir-279^{T:Disc\RFP-DsRed2} (Okamura et al., 2007) Rab5^{Scer\UAS.T:Disc\RFP-DsRed2} (Zhang et al., 2007) Rab7^{Scer\UAS.T:Disc\RFP-DsRed2} (Zhang et al., 2007) Rab9^{Scer\UAS.T:Disc\RFP-DsRed2} (Zhang et al., 2007) Rab11^{Scer\UAS.T:Disc\RFP-DsRed2} (Zhang et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	This is present in a series of vectors produced by Clontech.
Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	DsRed2 T:Disc\RFP^DsRed2
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Okamura et al., 2007, Cell 130(1): 89--100 The mirtron pathway generates microRNA-class regulatory RNAs in Drosophila. [FBrf0200988] Zhang et al., 2007, Genetics 176(2): 1307--1322 Thirty-one flavors of Drosophila Rab proteins. [FBrf0201049] Guan et al., 2006, Proc. Natl. Acad. Sci. USA 103(45): 16794--16799 Mutation of TweedleD, a member of an unconventional cuticle protein family, alters body shape in Drosophila. [FBrf0195309] Lai et al., 2005, Genes Dev. 19(9): 1067--1080 Pervasive regulation of Drosophila Notch target genes by GY-box-, Brd-box-, and K-box-class microRNAs. [FBrf0187594] Ronshaugen et al., 2005, Genes Dev. 19(24): 2947--2952 The Drosophila microRNA iab-4 causes a dominant homeotic transformation of halteres to wings. [FBrf0190560]
Erratum	Lai et al., 2005, Genes Dev. 19(11): 1400 Pervasive regulation of Drosophila Notch target genes by GY-box-, brd-box-, and K-box-class microRNAs (vol 19, pg 1067, 2005). [FBrf0187595]

Allele Disc\T:Disc\RFPDsRed2

Allele Disc\T:Disc\RFP^DsRed2