Allele Dmel\armi^72.1

General Information
Symbol	Dmel\armi^72.1	Species	D. melanogaster
Name		FlyBase ID	FBal0155636
Feature type	allele	Created / Updated	2006-08-22/2006-08-22
Associated gene	Dmel\armi

Allele class	loss of function
Mutagen	Δ2-3
Nature of the Allele
Allele class	loss of function (Cook et al., 2004)
Mutagen	Δ2-3 (Cook et al., 2004)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	armi¹ (Cook et al., 2004, Tomari et al., 2004)
Nature of the lesion	Statement Reference Imprecise excision of the P-element, resulting in a deletion of armi sequences in the 5' untranslated region. (Tomari et al., 2004)
Assay mode
Cytology
Phenotypic Data
Phenotypic Class
	female sterile (Cook et al., 2004) male sterile \| partially (Tomari et al., 2004) memory defective (with Df(3L)E1) (Ashraf et al., 2006) memory defective \| recessive (Ashraf et al., 2006)
Phenotype Manifest In
	dorsal appendage \| maternal effect (Cook et al., 2004, Tomari et al., 2004) egg \| maternal effect (Cook et al., 2004) oocyte & microtubule organizing center (Cook et al., 2004) oocyte & microtubule (Cook et al., 2004) dorsal appendage (with armi¹) (Klattenhoff et al., 2007) microtubule organizing center & oocyte (with armi¹) (Klattenhoff et al., 2007)
Detailed Description
	Statement Reference 92% of eggs laid by armi^72.1 females completely lack dorsal appendages (indicating strong dorsal/ventral patterning defects) and 35% of these are also collapsed. The anterior microtubule organising center (MTOC) forms in stage 1 armi^72.1 oocytes. However, during stages 2-7 a posterior MTOC is not apparent in the mutant oocyte and microtubule levels in the oocyte and nurse cells are comparable, in contrast to wild-type egg chambers where the oocyte is enriched in microtubules compared to the nurse cells during these stages. Cortical microtubules persist at the posterior cortex of mid-oogenesis stage mutant oocytes, in contrast to wild type. (Cook et al., 2004) 45% of progeny derived from homozygous males mated to wild-type females hatch. 92% of eggs derived from armi^72.1 females lack dorsal appendages. (Tomari et al., 2004) armi[72.1]/armi[1] eggs show fused appendages in 67.6% of cases, no appendages in 28.9% of cases and the wild-type number of appendages in 3.5% of cases. In armi[72.1]/armi[1] oocytes, both the anterior and later posterior microtubule-organizing centers are much less prominent than in wild-type oocytes. (Klattenhoff et al., 2007) armi[72.1] and armi[72.1]/Df(3L)E1 flies have a deficient long-term olfactory memory but show no defects in short-term olfactory memory. (Ashraf et al., 2006)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement Reference armi^72.1/armi¹ has dorsal appendage phenotype, suppressible by lok^p6/lok^p6/lok^p6/lok^p6 (Klattenhoff et al., 2007) armi^72.1/armi¹ has microtubule organizing center & oocyte phenotype, suppressible by lok^p6/lok^p6/lok^p6/lok^p6 (Klattenhoff et al., 2007) armi^72.1/armi¹ has dorsal appendage phenotype, suppressible \| partially by mei-41^D3/mei-41^D3 (Klattenhoff et al., 2007) armi^72.1/armi¹ has microtubule organizing center & oocyte phenotype, suppressible by mei-41^D3/mei-41^D3 (Klattenhoff et al., 2007)
NOT suppressed by
Statement Reference armi^72.1/armi¹ has dorsal appendage phenotype, non-suppressible by mei-W68¹/mei-W68^k05603 (Klattenhoff et al., 2007)
Additional Comments
Genetic Interactions
Statement Reference armi^72.1 osk¹ double mutant ovaries have microtubule defects that are cytologically identical to the armi single mutant phenotype. (Cook et al., 2004) The fused dorsal appendage phenotype of armi[72.1]/armi[1] eggs is suppressed in lok[p6]; armi[72.1]/armi[1] eggs with 92% of double mutants showing wild-type appendage morphology. The mei-41[D3] mutation partially suppresses the fused dorsal appendage phenotype of armi[72.1]/armi[1] eggs; 56% of mei-41[D3]; armi[72.1]/armi[1] eggs show wild-type appendage morphology. The fused dorsal appendage phenotype of armi[72.1]/armi[1] eggs is not suppressed in mei-W68[1]/mei-W68[k05603]; armi[72.1]/armi[1] eggs. The microtubule-organizing center phenotype of armi[72.1]/armi[1] oocytes is suppressed by the lok[p6] mutation. The microtubule-organizing center phenotype of armi[72.1]/armi[1] oocytes is partially suppressed by the mei-41[D3] mutation. (Klattenhoff et al., 2007)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	armi^72.1 is rescued by Scer\GAL4^elav-C155/armi^{Scer\UAS.P\T.T:Avic\GFP-EGFP} (Ashraf et al., 2006)
Partially rescued by	armi^72.1 is partially rescued by Scer\GAL4^nos.PG/armi^{Scer\UAS.P\T.T:Avic\GFP-EGFP} (Cook et al., 2004)
Comments
Stocks ( 1 )
Bloomington	8544 w^*; armi^72.1/TM6C, Sb¹ Tb¹
Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	armi^72.1 (Klattenhoff et al., 2007, Ashraf et al., 2006, Vagin et al., 2006, Ashraf et al., 2006)
Name Synonym
Secondary FlyBase IDs

References ( 9 )
Research paper	Klattenhoff et al., 2007, Dev. Cell 12(1): 45--55 Drosophila rasiRNA pathway mutations disrupt embryonic axis specification through activation of an ATR/Chk2 DNA damage response. [FBrf0192439] Ashraf et al., 2006, Cell 124(1): 191--205 Synaptic protein synthesis associated with memory is regulated by the RISC pathway in Drosophila. [FBrf0189896] Vagin et al., 2006, Science 313(5785): 320--324 A distinct small RNA pathway silences selfish genetic elements in the germline. [FBrf0194520] Forstemann et al., 2005, PLoS Biol. 3(7): 1187--1201 Normal microRNA maturation and germ-line stem cell maintenance requires Loquacious, a double-stranded RNA-binding domain protein. [FBrf0188210] Cook et al., 2004, Cell 116(6): 817--829 The Drosophila SDE3 homolog armitage is required for oskar mRNA silencing and embryonic axis specification. [FBrf0174441] Perrini et al., 2004, Molec. Cell 15(3): 467--476 HP1 controls telomere capping, telomere elongation, and telomere silencing by two different mechanisms in Drosophila. [FBrf0180524] Tomari et al., 2004, Cell 116(6): 831--841 RISC assembly defects in the Drosophila RNAi mutant armitage. [FBrf0174442]
Supplementary material	Ashraf et al., 2006, Cell 124(1): Supplemental Data. [FBrf0198682] Vagin et al., 2006, Science 313(5785): [FBrf0198717]

Allele Dmel\armi72.1

Allele Dmel\armi^72.1