Allele Dmel\dlp^{Scer\UAS.T:Ivir\HA1}

General Information
Symbol	Dmel\dlp^{Scer\UAS.T:Ivir\HA1}	Species	D. melanogaster
Name		FlyBase ID	FBal0138279
Feature type	allele	Created / Updated	2006-08-20/2006-08-20
Associated gene	Dmel\dlp

Allele class
Mutagen	in vitro construct \| regulatory fusion, in vitro construct \| coding region fusion
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Giraldez et al., 2002, Franch-Marro et al., 2005) in vitro construct \| coding region fusion (Giraldez et al., 2002, Franch-Marro et al., 2005)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of dlp tagged with T:Ivir\HA1. (Giraldez et al., 2002) Construct: Scer\UAS sequences drive expression of dlp tagged with T:Ivir\HA1. (Franch-Marro et al., 2005)
Assay mode	In transgenic Drosophila (intraspecific) (Giraldez et al., 2002, Franch-Marro et al., 2005) Drosophila cell culture (Franch-Marro et al., 2005)
Carried in construct	P{UAS-dlp.HA} (Rawson, 2005, Franch-Marro et al., 2005, Rawson et al., 2005, Hufnagel et al., 2006, Giraldez et al., 2002, Kreuger et al., 2004, Piddini et al., 2005)
Tagged with	T:Ivir\HA1 (Giraldez et al., 2002, Rawson et al., 2005, Franch-Marro et al., 2005, Piddini et al., 2005, Rawson, 2005)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^salm-459.2 (Giraldez et al., 2002)
Phenotype Manifest In
	wing, with Scer\GAL4^salm-459.2 (Giraldez et al., 2002) wing margin, with Scer\GAL4^en-e16E (Franch-Marro et al., 2005) wing margin \| posterior, with Scer\GAL4^en-e16E (Kreuger et al., 2004) wing margin bristle \| posterior, with Scer\GAL4^en-e16E (Kreuger et al., 2004) wing margin, with Scer\GAL4^dpp.blk1 (Franch-Marro et al., 2005) wing margin bristle \| cell non-autonomous \| ectopic \| somatic clone, with Scer\GAL4^αTub84B.PP (Franch-Marro et al., 2005)
Detailed Description
	Statement Reference Expression of dlp^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^salm-459.2 results in scalloping of the wing. (Giraldez et al., 2002) When dlp^{Scer\UAS.T:Ivir\HA1} is driven by Scer\GAL4^en-e16E or Scer\GAL4^dpp.blk1 there is a loss of wing margin tissue. When is driven in wing clones by Scer\GAL4^αTub84B.PP ectopic wing margin bristles are seen next to clones. (Franch-Marro et al., 2005) Notum[Scer\UAS.cGa]; Scer\GAL4[en-e16E] flies lack some posterior wing margin bristles. (Kreuger et al., 2004)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Scer\GAL4^elav-C155/dlp^{Scer\UAS.T:Ivir\HA1} is a suppressor \| partially of neuroanatomy defective phenotype of Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ (Rawson et al., 2005)
NOT Suppressor of
Statement Reference Scer\GAL4^elav-C155/dlp^{Scer\UAS.T:Ivir\HA1} is a non-suppressor of neurophysiology defective phenotype of Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ (Rawson et al., 2005)
Phenotype Manifest In
Enhanced by
Statement Reference dlp^{Scer\UAS.T:Ivir\HA1} has wing margin \| posterior phenotype, enhanceable by Scer\GAL4^en-e16E/Notum^Scer\UAS.cGa (Kreuger et al., 2004)
Enhancer of
Statement Reference Scer\GAL4^en-e16E/dlp^{Scer\UAS.T:Ivir\HA1} is an enhancer of wing margin \| posterior phenotype of Notum^Scer\UAS.cGa (Kreuger et al., 2004)
Suppressor of
Statement Reference Scer\GAL4^elav-C155/dlp^{Scer\UAS.T:Ivir\HA1} is a suppressor of medulla phenotype of Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ (Rawson et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference Expression of dlp^{Scer\UAS.T:Ivir\HA1}, under the control of Scer\GAL4^elav-C155, rescues the medulla patterning defects of Sdc¹⁰⁶⁰⁸/Df(2R)48 mutants (in which Sara function has been rescued by Sara^Ubi.PJ). However, this expression fails to rescue the ERG defects. (Rawson et al., 2005) Notum[Scer\UAS.cGa]; dlp[Scer\UAS.T:Ivir\HA1] Scer\GAL4[en-e16E] have a scalloped posterior wing margin. (Kreuger et al., 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	dlp^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^ey.PH rescues dlp¹ (Rawson et al., 2005) dlp^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4[-] rescues dlp² (Rawson et al., 2005) dlp^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^ey.PH rescues dlp² (Rawson et al., 2005) dlp^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4[-] rescues dlp¹ (Rawson et al., 2005)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Carried in a plasmid and transfected into S2 cells. (Franch-Marro et al., 2005)
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	dlp^{Scer\UAS.T:Ivir\HA1}
Name Synonym
Secondary FlyBase IDs

References ( 7 )
Research paper	Hufnagel et al., 2006, Dev. Biol. 300(2): 512--522 On the role of glypicans in the process of morphogen gradient formation. [FBrf0195028] Franch-Marro et al., 2005, Development 132(4): 659--666 Glypicans shunt the Wingless signal between local signalling and further transport. [FBrf0187459] Piddini et al., 2005, Development 132(24): 5479--5489 Arrow (LRP6) and Frizzled2 cooperate to degrade Wingless in Drosophila imaginal discs. [FBrf0190314] Rawson et al., 2005, Curr. Biol. 15(9): 833--838 The heparan sulfate proteoglycans daily-like and syndecan have distinct functions in axon guidance and visual-system assembly in Drosophila. [FBrf0187328] Kreuger et al., 2004, Dev. Cell 7(4): 503--512 Opposing activities of Dally-like glypican at high and low levels of Wingless morphogen activity. [FBrf0180125] Giraldez et al., 2002, Dev. Cell 2(5): 667--676 HSPG modification by the secreted enzyme Notum shapes the Wingless morphogen gradient. [FBrf0148946]
Supplementary material	Rawson, 2005, Curr. Biol. 15(9): Document S1. Experimental Procedures and Four Figures. [FBrf0191734]

Allele Dmel\dlpScer\UAS.T:Ivir\HA1

Allele Dmel\dlp^{Scer\UAS.T:Ivir\HA1}