Allele Dmel\Fmr1^Scer\UAS.cZa

General Information
Symbol	Dmel\Fmr1^Scer\UAS.cZa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Zhang	FlyBase ID	FBal0131027
Feature type	allele	Created / Updated	2006-08-20/2006-08-20
Associated gene	Dmel\Fmr1

Allele class
Mutagen	in vitro construct \| regulatory fusion
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Zhang et al., 2001)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of Fmr1 is governed by Scer\UAS regulatory sequences. (Zhang et al., 2001)
Assay mode	In transgenic Drosophila (intraspecific) (Zhang et al., 2001)
Carried in construct	P{UAS-Fmr1.Z} (Reeve et al., 2005, Zhang et al., 2001, Morales et al., 2002, Pan et al., 2004)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| pupal stage, with Scer\GAL4^G7 (Zhang et al., 2001) lethal \| adult stage, with Scer\GAL4^elav-C155 (Zhang et al., 2001) lethal, with Fmr1^{I307N.Z.Scer\UAS}, Scer\GAL4^elav-C155 (Zhang et al., 2001) visible, with Scer\GAL4^elav-C155 (Zhang et al., 2001) uncoordinated, with Scer\GAL4^elav-C155 (Zhang et al., 2001) flightless, with Scer\GAL4^elav-C155 (Zhang et al., 2001) flightless, with Scer\GAL4^Mhc.PW (Zhang et al., 2001) neurophysiology defective, with Scer\GAL4^hs.2sev (Zhang et al., 2001) neuroanatomy defective \| somatic clone, with Scer\GAL4^OK107 (Pan et al., 2004) neuroanatomy defective, with Scer\GAL4^elav-C155 (Zhang et al., 2001) neuroanatomy defective, with Scer\GAL4^OK107 (Pan et al., 2004) neuroanatomy defective, with Scer\GAL4^Mhc.PW (Zhang et al., 2001) neuroanatomy defective, with Scer\GAL4^c747 (Reeve et al., 2005) neuroanatomy defective, with Scer\GAL4^P2.4.Pdf (Reeve et al., 2005) neuroanatomy defective, with Scer\GAL4^ato.3.6 (Morales et al., 2002)
Phenotype Manifest In
	dorsal cluster neuron, with Scer\GAL4^ato.3.6 (Morales et al., 2002) beta'-lobe, with Scer\GAL4^OK107 (Pan et al., 2004) beta-lobe, with Scer\GAL4^OK107 (Pan et al., 2004) gamma-lobe, with Scer\GAL4^OK107 (Pan et al., 2004) mushroom body & neuron \| somatic clone, with Scer\GAL4^OK107 (Pan et al., 2004) mushroom body & neuron & dendrite \| somatic clone, with Scer\GAL4^OK107 (Pan et al., 2004) gamma-lobe & neuron \| somatic clone, with Scer\GAL4^OK107 (Pan et al., 2004) wing, with Scer\GAL4^elav-C155 (Zhang et al., 2001) neuromuscular junction, with Scer\GAL4^elav-C155 (Zhang et al., 2001) bouton, with Scer\GAL4^elav-C155 (Zhang et al., 2001) wing, with Scer\GAL4^Mhc.PW (Zhang et al., 2001) neuromuscular junction, with Scer\GAL4^Mhc.PW (Zhang et al., 2001) retina, with Scer\GAL4^hs.2sev (Zhang et al., 2001) rhabdomere, with Scer\GAL4^hs.2sev (Zhang et al., 2001) eye photoreceptor cell, with Scer\GAL4^hs.2sev (Zhang et al., 2001) ommatidium, with Scer\GAL4^hs.2sev (Zhang et al., 2001) photoreceptor cell & synapse & lamina receptor cell, with Scer\GAL4^hs.2sev (Zhang et al., 2001) adult mushroom body, with Scer\GAL4^c747 (Reeve et al., 2005) ventral adult lateral neuron, with Scer\GAL4^P2.4.Pdf (Reeve et al., 2005)
Detailed Description
	Statement Reference When expression is driven by Scer\GAL4^elav-C155 flies show abnormally outspread wings and cannot fly. Flies are uncoordinated and show adult lethality - dying 5-10 days post-eclosion. When expression is driven by Scer\GAL4^Mhc.PW flies' wings are droopy or held up and they cannot fly. When expression is driven by Scer\GAL4^G7 flies show pupal lethality. When expression is driven by Scer\GAL4^hs.2sev the retina is disordered with misshapen rhabdomeres, abnormal rhabdomere numbers and fused ommatidia. Phototransduction is normal, however synaptic transmission is reduced (as indicated by a reduction in off-transient mean amplitude as assayed by ERG). When expression is driven by Scer\GAL4^elav-C155 the larval neuromuscular junction shows pronounced synaptic undergrowth and underelaboration of synaptic terminals. Muscle 4 has 36% decrease in number of boutons over controls. Bouton diameter is nearly twice that of wild type. When expression is driven by Scer\GAL4^Mhc.PW the larval neuromuscular junction shows pronounced synaptic undergrowth and underelaboration of synaptic terminals. Muscle 4 has 17% decrease in number of boutons over controls. Mean EJC amplitude is unaffected when expression is driven by Scer\GAL4^elav-C155 but mEJC analysis shows that the frequency of spontaneous glutamate release is increased five-fold (though unaffected when driven by Scer\GAL4^Mhc.PW). (Zhang et al., 2001) When expression is driven by Scer\GAL4^ato.3.6 axon extension from the lobula to the medulla fails completely. Fibers enter the lobula but then either project to inappropriate sites within the lobula or stop at the lobula-medulla border. (Morales et al., 2002) Expression of Fmr1^Scer\UAS.cZa under the control of Scer\GAL4^OK107 results in dramatic defects in the mushroom body axon lobes; the β lobe always overextends across the midline, the β' lobe often shows apparently random directions of axon projection which are often outside of the mushroom body domain and the γ lobe always shows a dramatic decrease in volume and is sometimes completely lost. The number of cell body processes produced by neuronal cells in the mushroom body is reduced 2-fold in single cell clones expressing Fmr1^Scer\UAS.cZa under the control of Scer\GAL4^OK107 compared to control single cell clones. The "claw-like" structure normally seen at the terminal of dendritic branches (this consists of a fine dendritic terminal arbor) is severely reduced or completely absent in the mutant cells. Single γ neurons in the mushroom body that express Fmr1^Scer\UAS.cZa under the control of Scer\GAL4^OK107 in an otherwise wild-type background show underbranching and dramatically reduced growth. Most of these mutant γ neurons maintain their process along the ventral edge of the horizontal lobe and aberrantly invade the β lobe. (Pan et al., 2004) Flies that express Fmr1^Scer\UAS.cZa, under the control of Scer\GAL4^P2.4.Pdf, almost completely lack wild-type target innervation by the LNv. In the brains of flies that overexpress Fmr1^Scer\UAS.cZa under the control of Scer\GAL4^c747, there is a significant increase in the gap between the β lobes and between the γ lobes of the mushroom body. (Reeve et al., 2005)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Fmr1^Scer\UAS.cZa, Scer\GAL4^c747 has neuroanatomy defective phenotype, suppressible by chic^Scer\UAS.cCa, Scer\GAL4^c747 (Reeve et al., 2005) Fmr1^Scer\UAS.cZa, Scer\GAL4^c747 has neuroanatomy defective phenotype, suppressible by chic^Scer\UAS.cCa/Scer\GAL4^P2.4.Pdf (Reeve et al., 2005)
Suppressor of
Statement Reference Fmr1^Scer\UAS.cZa, Scer\GAL4^P2.4.Pdf is a suppressor of neuroanatomy defective phenotype of Scer\GAL4^P2.4.Pdf, chic^Scer\UAS.cCa (Reeve et al., 2005)
Phenotype Manifest In
Suppressed by
Statement Reference Fmr1^Scer\UAS.cZa, Scer\GAL4^c747 has adult mushroom body phenotype, suppressible by chic^Scer\UAS.cCa, Scer\GAL4^c747 (Reeve et al., 2005) Fmr1^Scer\UAS.cZa, Scer\GAL4^c747 has ventral adult lateral neuron phenotype, suppressible by chic^Scer\UAS.cCa/Scer\GAL4^P2.4.Pdf (Reeve et al., 2005)
Suppressor of
Statement Reference Fmr1^Scer\UAS.cZa, Scer\GAL4^P2.4.Pdf is a suppressor of ventral adult lateral neuron & commissure phenotype of Scer\GAL4^P2.4.Pdf, chic^Scer\UAS.cCa (Reeve et al., 2005) Fmr1^Scer\UAS.cZa, Scer\GAL4^P2.4.Pdf is a suppressor of ventral adult lateral neuron phenotype of Scer\GAL4^P2.4.Pdf, chic^Scer\UAS.cCa (Reeve et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference Flies that co-express chic^Scer\UAS.cCa and Fmr1^Scer\UAS.cZa, both under the control of Scer\GAL4^P2.4.Pdf, show a wild-type-like pattern of LNv innervation. The LNv fasciculation phenotype seen when chic^Scer\UAS.cCa is expressed alone is suppressed. Coexpression of chic^Scer\UAS.cCa significantly reduces the mushroom body phenotype seen when flies overexpress Fmr1^Scer\UAS.cZa under the control of Scer\GAL4^c747. (Reeve et al., 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	6931 w¹¹¹⁸; P{UAS-Fmr1.Z}3
Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	dfmr^OE (Pan et al., 2004) Fmr1^Scer\UAS.cZa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Zhang (Zhang et al., 2001)
Secondary FlyBase IDs

References ( 4 )
Research paper	Reeve et al., 2005, Curr. Biol. 15(12): 1156--1163 The Drosophila fragile X mental retardation protein controls actin dynamics by directly regulating profilin in the brain. [FBrf0187329] Pan et al., 2004, Curr. Biol. 14(20): 1863--1870 The Drosophila fragile X gene negatively regulates neuronal elaboration and synaptic differentiation. [FBrf0180069] Morales et al., 2002, Neuron 34(6): 961--972 Drosophila Fragile X protein, DFXR, regulates neuronal morphology and function in the brain. [FBrf0149142] Zhang et al., 2001, Cell 107(5): 591--603 Drosophila fragile X-related gene regulates the MAP1B homolog Futsch to control synaptic structure and function. [FBrf0141416]

Allele Dmel\Fmr1Scer\UAS.cZa

Allele Dmel\Fmr1^Scer\UAS.cZa