Allele Dmel\sr^b.Scer\UAS

General Information
Symbol	Dmel\sr^b.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0065677
Feature type	allele	Created / Updated	2006-08-20/2006-08-20
Associated gene	Dmel\sr

Allele class
Mutagen	in vitro construct \| regulatory fusion
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Vorbruggen and Jackle, 1997)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: An EcoRI/XbaI fragment of the sr b cDNA (nucleotides 405 to 4458) is expressed under the control of Scer\UAS regulatory sequences. (Vorbruggen and Jackle, 1997)
Assay mode	In transgenic Drosophila (intraspecific) (Vorbruggen and Jackle, 1997)
Carried in construct	P{UAS-sr.b.V} (Usui et al., 2004, Vorbruggen and Jackle, 1997)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^sca-C253 (Usui et al., 2004) visible, with Scer\GAL4^sr-md710 (Usui et al., 2004)
Phenotype Manifest In
	mesothoracic tergum & microchaeta, with Scer\GAL4^sr-md710 (Usui et al., 2004) mesothoracic tergum & chaeta, with Scer\GAL4^sca-C253 (Usui et al., 2004) embryonic/larval somatic muscle \| ventral, with Scer\GAL4^sim.PS (Vorbruggen and Jackle, 1997) embryonic/larval somatic muscle, with Scer\GAL4^en-e16E (Vorbruggen and Jackle, 1997) lateral transverse muscle & embryo, with Scer\GAL4^en-e16E (Vorbruggen and Jackle, 1997) embryonic/larval somatic muscle, with Scer\GAL4^ptc-559.1 (Vorbruggen and Jackle, 1997) abdominal 5 ventral longitudinal muscle & embryo, with Scer\GAL4^h.6 (Vorbruggen and Jackle, 1997) abdominal 6 ventral longitudinal muscle & embryo, with Scer\GAL4^h.6 (Vorbruggen and Jackle, 1997) abdominal 7 ventral longitudinal muscle & embryo, with Scer\GAL4^h.6 (Vorbruggen and Jackle, 1997) abdominal 5 lateral transverse muscle & embryo, with Scer\GAL4^h.6 (Vorbruggen and Jackle, 1997) abdominal 6 lateral transverse muscle & embryo, with Scer\GAL4^h.6 (Vorbruggen and Jackle, 1997) abdominal 7 lateral transverse muscle & embryo, with Scer\GAL4^h.6 (Vorbruggen and Jackle, 1997) embryonic/larval somatic muscle, with Scer\GAL4^hs.2sev (Vorbruggen and Jackle, 1997)
Detailed Description
	Statement Reference Expression of sr^b.Scer\UAS in the developing mesoderm using Scer\GAL4^how-24B does not cause muscle defects. Expression of sr^b.Scer\UAS in the ventral midline using Scer\GAL4^sim.PS results in strong distortion of the ventral muscles, which frequently cross the midline. Expression of sr^b.Scer\UAS using Scer\GAL4^en-e16E produces a disturbed muscle pattern, and lateral transverse muscles with abnormally elongated processes are seen. Expression of sr^b.Scer\UAS using Scer\GAL4^ptc-559.1 produces a disturbed muscle pattern, and fused myotubes of abnormal shape are seen. Expression of sr^b.Scer\UAS using Scer\GAL4^h.6 results in the absence of ventral longitudinal and lateral transversal muscles in abdominal segments 5 to 7. Ubiquitous expression of sr^b.Scer\UAS using Scer\GAL4^hs.2sev results in an abnormal muscle pattern only when expression occurs prior to embryonic stage 15. When expressed at stage 12 using Scer\GAL4^hs.2sev, abnormal outgrowth of muscles is seen. (Vorbruggen and Jackle, 1997) Expression of sr^b.Scer\UAS under the control of Scer\GAL4^sr-md710 results in a severe decrease in the number of microchaetae on the notum. Expression of sr^b.Scer\UAS under the control of Scer\GAL4^sca-C253 results in a total lack of bristles on the notum. (Usui et al., 2004)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference sr^b.Scer\UAS/Scer\GAL4^sca-C253 is a suppressor of visible phenotype of Scer\GAL4^sca-C253, sc^Scer\UAS.cHa (Usui et al., 2004)
Phenotype Manifest In
Suppressor of
Statement Reference sr^b.Scer\UAS/Scer\GAL4^sca-C253 is a suppressor of mesothoracic tergum & macrochaeta \| ectopic phenotype of Scer\GAL4^sca-C253, sc^Scer\UAS.cHa (Usui et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of sr^b.Scer\UAS removes most of the ectopic bristles on the notum caused by expression of sc^Scer\UAS.cHa under the control of Scer\GAL4^sca-C253 and also removes many of the extant ones. (Usui et al., 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	sr^b.Scer\UAS sr^b.UAS
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Usui et al., 2004, Curr. Biol. 14(12): 1047--1055 Mutual exclusion of sensory bristles and tendons on the notum of dipteran flies. [FBrf0179495] Vorbruggen and Jackle, 1997, Proc. Natl. Acad. Sci. USA 94(16): 8606--8611 Epidermal muscle attachment site-specific target gene expression and interference with myotube guidance in response to ectopic stripe expression in the developing Drosophila epidermis. [FBrf0097704]

Allele Dmel\srb.Scer\UAS

Allele Dmel\sr^b.Scer\UAS