Allele Dmel\N^Scer\UAS.cSa

General Information
Symbol	Dmel\N^Scer\UAS.cSa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Seugnet	FlyBase ID	FBal0048889
Feature type	allele	Created / Updated	2006-08-20/2006-08-20
Associated gene	Dmel\N

Allele class
Mutagen	in vitro construct \| regulatory fusion
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Doherty et al., 1996, Frise et al., 1996)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of N coding region is governed by Scer\UAS regulatory sequences. (Doherty et al., 1996) N expression is under the control of Scer\UAS regulatory sequences. (Frise et al., 1996)
Assay mode	In transgenic Drosophila (intraspecific) (Frise et al., 1996, Doherty et al., 1996)
Carried in construct	P{UAS-N.S} (Younger, 2001.2, Li et al., 2003, Doherty et al., 1997, Doherty et al., 1996, Chandra et al., 2003, Guo et al., 1996, Frise et al., 1996, Seugnet et al., 1997, Ward et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| larval stage, with Scer\GAL4^109-79 (Younger, 2001.2) lethal \| larval stage, with Scer\GAL4^109-53 (Younger, 2001.2) lethal \| larval stage, with Scer\GAL4^109-39 (Younger, 2001.2) lethal \| larval stage, with Scer\GAL4^109-30 (Younger, 2001.2) visible, with Scer\GAL4^sca-537.4 (Doherty et al., 1997) visible, with Scer\GAL4^pnr-MD237 (Seugnet et al., 1997) visible, with Scer\GAL4^sca-109-68 (Doherty et al., 1997) visible, with Scer\GAL4^410.2 (Seugnet et al., 1997)
Phenotype Manifest In
	thorax \| dorsal & macrochaeta, with Scer\GAL4^pnr-MD237 (Chandra et al., 2003) trichogen cell, with Scer\GAL4^pnr-MD237 (Chandra et al., 2003) tormogen cell \| ectopic, with Scer\GAL4^pnr-MD237 (Chandra et al., 2003) microchaeta \| precursor, with Scer\GAL4^pnr-MD237 (Seugnet et al., 1997) wing & microchaeta, with Scer\GAL4^ptc-559.1 (Doherty et al., 1996) microchaeta \| ectopic, with Scer\GAL4^ptc-559.1 (Doherty et al., 1996) wing, with Scer\GAL4^ptc-559.1 (Doherty et al., 1996) wing vein \| ectopic, with Scer\GAL4^dpp.blk1 (Lee et al., 2000) anterior crossvein, with Scer\GAL4^dpp.blk1 (Lee et al., 2000) macrochaeta, with Scer\GAL4^sca-109-68 (Younger, 2001.2) socket, with Scer\GAL4^sca-109-68 (Frise et al., 1996) photoreceptor cell R8, with Scer\GAL4^sca-109-68 (Li et al., 2003) eye, with Scer\GAL4^sca-109-68 (Li et al., 2003) sense organ, with Scer\GAL4^sca-109-68 (Doherty et al., 1997) trichogen cell, with Scer\GAL4^sca-109-68 (Guo et al., 1996) microchaeta \| precursor, with Scer\GAL4^410.2 (Seugnet et al., 1997) sense organ, with Scer\GAL4^sca-537.4 (Doherty et al., 1997) neuron, with Scer\GAL4¹⁴⁰⁷ (Guo et al., 1996)
Detailed Description
	Statement Reference When expression is driven by Scer\GAL4^ptc-559.1, flies show a short row of ectopic hairs along the fourth wing vein on both dorsal and ventral sides of the wing blade, similar to anterior double row or posterior wing margin cells. Occasional wing outgrowth is observed. (Doherty et al., 1996) Scer\GAL4¹⁴⁰⁷ induced expression causes mild transformation of neurons to sheath cells. Scer\GAL4^sca-109-68 induced expression causes 20% of the bristles in the notum to form double sockets, or less frequently triple or four sockets. (Guo et al., 1996) When expressed in the sensory organ precursor cells of the adult external sense organs, using Scer\GAL4^sca-109-68, a double socket phenotype is produced. (Frise et al., 1996) N^Scer\UAS.cSa causes loss of sense organs and rare hair-to-socket transformations when expressed using Scer\GAL4^sca-537.4 or Scer\GAL4^sca-109-68. (Doherty et al., 1997) Scer\GAL4^pnr-MD237-mediated expression can cause loss of bristles. Scer\GAL4^pnr-MD237- or Scer\GAL4^410.2-mediated expression suppresses the development of neural precursors in a shi mutant background. (Seugnet et al., 1997) The expression of N^Scer\UAS.cSa when driven by Scer\GAL4^dpp.blk1 causes a thickening of wing veins and a reduction of the anterior crossvein. (Lee et al., 2000) When driven by Scer\GAL4^sca-109-68 bristle defects occur. (Younger, 2001.2) When N^Scer\UAS.cSa is driven by Scer\GAL4^pnr-MD237 most of the bristles are lost from the dorsal-most region of the thorax. In addition, occasionally, bristle to socket transformation is observed. (Chandra et al., 2003) When N^Scer\UAS.cSa is driven by Scer\GAL4^sca-109-68 R8 photoreceptor cells are sometimes missing. Adult eyes are small and rough. (Li et al., 2003)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference N^Scer\UAS.cSa, Scer\GAL4^sca-109-68 has visible phenotype, enhanceable by bib^Scer\UAS.cDa, Scer\GAL4^sca-109-68 (Doherty et al., 1997) N^Scer\UAS.cSa, Scer\GAL4^sca-537.4 has visible phenotype, enhanceable by bib^Scer\UAS.cDa, Scer\GAL4^sca-537.4 (Doherty et al., 1997)
Enhancer of
Statement Reference N^Scer\UAS.cSa, Scer\GAL4^sca-109-68 is an enhancer of visible phenotype of Scer\GAL4^sca-109-68, bib^Scer\UAS.cDa (Doherty et al., 1997) N^Scer\UAS.cSa, Scer\GAL4^sca-537.4 is an enhancer of visible phenotype of Scer\GAL4^sca-537.4, bib^Scer\UAS.cDa (Doherty et al., 1997)
Suppressor of
Statement Reference N^Scer\UAS.cSa, Scer\GAL4^bbg-C96 is a suppressor of visible phenotype of Nsf2^EQ.Scer\UAS, Scer\GAL4^bbg-C96 (Stewart et al., 2001)
Phenotype Manifest In
Enhanced by
Statement Reference N^Scer\UAS.cSa, Scer\GAL4^sca-109-68 has sense organ phenotype, enhanceable by bib^Scer\UAS.cDa, Scer\GAL4^sca-109-68 (Doherty et al., 1997) N^Scer\UAS.cSa, Scer\GAL4^sca-537.4 has sense organ phenotype, enhanceable by bib^Scer\UAS.cDa, Scer\GAL4^sca-537.4 (Doherty et al., 1997)
NOT Enhanced by
Statement Reference N^Scer\UAS.cSa, Scer\GAL4^dpp.blk1 has anterior crossvein phenotype, non-enhanceable by sca^Scer\UAS.cEa, Scer\GAL4^dpp.blk1 (Lee et al., 2000) N^Scer\UAS.cSa, Scer\GAL4^dpp.blk1 has wing vein \| ectopic phenotype, non-enhanceable by sca^Scer\UAS.cEa, Scer\GAL4^dpp.blk1 (Lee et al., 2000)
Suppressed by
Statement Reference N^Scer\UAS.cSa, Scer\GAL4^pnr-MD237 has macrochaeta \| ectopic phenotype, suppressible \| partially by fred^{dsRNA.Scer\UAS}, Scer\GAL4^pnr-MD237 (Chandra et al., 2003) N^Scer\UAS.cSa, Scer\GAL4^pnr-MD237 has mesothoracic tergum phenotype, suppressible \| partially by fred^{dsRNA.Scer\UAS}, Scer\GAL4^pnr-MD237 (Chandra et al., 2003)
NOT suppressed by
Statement Reference N^Scer\UAS.cSa, Scer\GAL4^dpp.blk1 has anterior crossvein phenotype, non-suppressible by sca^Scer\UAS.cEa, Scer\GAL4^dpp.blk1 (Lee et al., 2000) N^Scer\UAS.cSa, Scer\GAL4^dpp.blk1 has wing vein \| ectopic phenotype, non-suppressible by sca^Scer\UAS.cEa, Scer\GAL4^dpp.blk1 (Lee et al., 2000)
Enhancer of
Statement Reference N^Scer\UAS.cSa, Scer\GAL4^sca-109-68 is an enhancer of sense organ phenotype of Scer\GAL4^sca-109-68, bib^Scer\UAS.cDa (Doherty et al., 1997) N^Scer\UAS.cSa, Scer\GAL4^sca-537.4 is an enhancer of sense organ phenotype of Scer\GAL4^sca-537.4, bib^Scer\UAS.cDa (Doherty et al., 1997)
Suppressor of
Statement Reference N^Scer\UAS.cSa, Scer\GAL4^bbg-C96 is a suppressor of wing margin phenotype of Nsf2^EQ.Scer\UAS, Scer\GAL4^bbg-C96 (Stewart et al., 2001) N^Scer\UAS.cSa, Scer\GAL4^dpp.blk1 is a suppressor of wing \| distal phenotype of Scer\GAL4^dpp.blk1, sca^Scer\UAS.cEa (Lee et al., 2000) N^Scer\UAS.cSa, Scer\GAL4^pnr-MD237 is a suppressor \| partially of macrochaeta \| ectopic phenotype of Scer\GAL4^pnr-MD237, fred^{dsRNA.Scer\UAS} (Chandra et al., 2003) N^Scer\UAS.cSa, Scer\GAL4^pnr-MD237 is a suppressor \| partially of mesothoracic tergum phenotype of Scer\GAL4^pnr-MD237, fred^{dsRNA.Scer\UAS} (Chandra et al., 2003)
Additional Comments
Genetic Interactions
Statement Reference N^Scer\UAS.cSa interacts synergistically with bib^Scer\UAS.cDa, producing increased sense organ loss and more frequent hair-to-socket transformations when they are expressed using Scer\GAL4^sca-537.4 or Scer\GAL4^sca-109-68. (Doherty et al., 1997) When fred^{dsRNA.Scer\UAS} and N^Scer\UAS.cSa are both driven by Scer\GAL4^pnr-MD237, a phenotype that is intermediate between the two individual phenotypes. (Chandra et al., 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer	L. Seugnet, M. Haenlin and P. Simpson.

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	N^Scer\UAS.cSa (Ward et al., 2006) N^UAS.cSa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Seugnet (Doherty et al., 1996)
Secondary FlyBase IDs

References ( 11 )
Research paper	Ward et al., 2006, Dev. Biol. 297(2): 461--470 Border of Notch activity establishes a boundary between the two dorsal appendage tube cell types. [FBrf0200192] Chandra et al., 2003, Dev. Biol. 256(2): 302--316 The Drosophila IgC2 domain protein Friend-of-Echinoid, a paralogue of Echinoid, limits the number of sensory organ precursors in the wing disc and interacts with the Notch signaling pathway. [FBrf0158849] Li et al., 2003, Development 130(13): 2829--2840 Notch activity in neural cells triggered by a mutant allele with altered glycosylation. [FBrf0160738] Stewart et al., 2001, Dev. Biol. 234(1): 13--23 Snare-dependent signaling at the Drosophila wing margin. [FBrf0135720] Lee et al., 2000, Curr. Biol. 10(15): 931--934 The scabrous protein can act as an extracellular antagonist of notch signaling in the Drosophila wing. [FBrf0129929] Doherty et al., 1997, Development 124(19): 3881--3893 The Drosophila neurogenic gene big brain, which encodes a membrane-associated protein, acts cell autonomously and can act synergistically with Notch and Delta. [FBrf0099033] Seugnet et al., 1997, Dev. Biol. 192(2): 585--598 Requirement for dynamin during Notch signaling in Drosophila neurogenesis. [FBrf0100241] Doherty et al., 1996, Genes Dev. 10(4): 421--434 Delta is a ventral to dorsal signal complementary to serrate, another Notch ligand, in Drosophila wing formation. [FBrf0086385] Frise et al., 1996, Proc. Natl. Acad. Sci. USA 93(21): 11925--11932 The Drosophila Numb protein inhibits signaling of the Notch receptor during cell-cell interaction in sensory organ lineage. [FBrf0090560] Guo et al., 1996, Neuron 17(1): 27--41 Control of daughter cell fates during asymmetric division: Interaction of numb and Notch. [FBrf0089977]
Personal communication to FlyBase	Younger, 2001.2, Younger P{GawB} insertions. Younger P{GawB} insertions. [FBrf0159894]

Allele Dmel\NScer\UAS.cSa

Allele Dmel\N^Scer\UAS.cSa