Allele Dmel\gbb^Scer\UAS.cSb

General Information
Symbol	Dmel\gbb^Scer\UAS.cSb	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct b of Staehling-Hampton	FlyBase ID	FBal0042786
Feature type	allele	Created / Updated	2006-08-20/2006-08-20
Associated gene	Dmel\gbb

Allele class
Mutagen	in vitro construct \| regulatory fusion
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Staehling-Hampton et al., 1994)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: gbb EcoRI fragment cDNA (FBrf0055818), without an SV40 poly(A) signal, is under the transcriptional control of Scer\GAL4 UAS binding sites. (Staehling-Hampton et al., 1994)
Assay mode	In transgenic Drosophila (intraspecific) (Staehling-Hampton et al., 1994)
Carried in construct	P{UAS-Tgfβ-60A.S2} (Staehling-Hampton et al., 1994, Wharton, 2003.10.8, Miguel-Aliaga et al., 2004, Allan et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| pupal stage, with Scer\GAL4^how-24B (Staehling-Hampton et al., 1994) visible, with Scer\GAL4^dpp.blk1 (Staehling-Hampton et al., 1994)
Phenotype Manifest In
	wing, with Scer\GAL4^dpp.blk1 (Staehling-Hampton et al., 1994)
Detailed Description
	Statement Reference Expression of P{GawB}69B causes pupal lethality, adult escapers exhibit a distinctive deflated wing phenotype. Expression of P{GAL4-dpp.blk1} causes wing blistering. (Staehling-Hampton et al., 1994)
Interactions
Phenotypic Class
Phenotype Manifest In
NOT Suppressor of
Statement Reference gbb^Scer\UAS.cSb/Scer\GAL4^dpp.blk1 is a non-suppressor of eye phenotype of dpp^d6 (Staehling-Hampton et al., 1994) gbb^Scer\UAS.cSb/Scer\GAL4^dpp.blk1 is a non-suppressor of wing phenotype of dpp^d6 (Staehling-Hampton et al., 1994) gbb^Scer\UAS.cSb/Scer\GAL4^dpp.blk1 is a non-suppressor of eye phenotype of dpp^d-blk (Staehling-Hampton et al., 1994)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to rescue	Scer\GAL4^unspecified/gbb^Scer\UAS.cSb fails to rescue gbb^unspecified (Wharton, 2003.10.8)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	UAS-gbb lines that have the SV40 poly(A) signal produce weaker phenotypes than the lines with the signal removed, when crossed to different Scer\GAL4 lines. P{GAL4-dpp.blk1} driven expression of P{UAS-Tgfβ-60A.S1} or P{UAS-Tgfβ-60A.S2} is unable to rescue the mutant phenotype of dpp^d-blk or dpp^d6. gbb cannot substitute dpp function in the imaginal discs. (Staehling-Hampton et al., 1994)
Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	gbb^Scer\UAS.cSb gbb^UAS.cSb Tgfβ-60A^UAS.cSb
Name Synonym	Saccharomyces cerevisiae UAS construct b of Staehling-Hampton (Staehling-Hampton et al., 1994)
Secondary FlyBase IDs

References ( 4 )
Research paper	Miguel-Aliaga et al., 2004, Development 131(23): 5837--5848 Independent roles of the dachshund and eyes absent genes in BMP signaling, axon pathfinding and neuronal specification. [FBrf0180178] Allan et al., 2003, Cell 113(1): 73--86 Specification of neuropeptide cell identity by the integration of retrograde BMP signaling and a combinatorial transcription factor code. [FBrf0158766] Staehling-Hampton et al., 1994, Cell Growth Diffn 5(6): 585--593 Specificity of bone morphogenetic protein-related factors: Cell fate and gene expression changes in Drosophila embryos induced by decapentaplegic but not 60A. [FBrf0074522]
Personal communication to FlyBase	Wharton, 2003.10.8, UAS-gbb lines. UAS-gbb lines. [FBrf0173062]

Allele Dmel\gbbScer\UAS.cSb

Allele Dmel\gbb^Scer\UAS.cSb