Allele Dmel\aub^QC42

General Information
Symbol	Dmel\aub^QC42	Species	D. melanogaster
Name		FlyBase ID	FBal0030533
Feature type	allele	Created / Updated	2006-08-22/2006-08-22
Associated gene	Dmel\aub

Allele class
Mutagen	ethyl methanesulfonate
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Schupbach and Wieschaus, 1991)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Assay mode
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| maternal effect \| recessive (Wilson et al., 1996) female sterile (Schupbach and Wieschaus, 1991) suppressor of variegation (Pal-Bhadra et al., 2004) suppressor of variegation, with P{T1}T190-177 (Haynes et al., 2006) suppressor of variegation, with P{T1(-FRT)}T190-177 (Haynes et al., 2006)
Phenotype Manifest In
	embryo \| maternal effect \| posterior (Wilson et al., 1996) dorsal appendage (with aub^HN) (Klattenhoff et al., 2007)
Detailed Description
	Statement Reference Most extreme eggs are spindle shaped without dorsal appendages. Other eggs have fused dorsal appendages or of normal morphology but remain unfertilized. (Schupbach and Wieschaus, 1991) 2% of eggs from homozygous mothers are fertilized and embryos lack abdominal segments and pole cells, i.e. show a classic posterior group mutant phenotype. (Wilson et al., 1996) Suppressor of repeat-induced silencing, as seen at heterochromatic tandem arrays of insertions of the P{lacW} transposon. (Pal-Bhadra et al., 2004) aub[HN]/aub[QC42] eggs show fused dorsal appendages in 40% of cases, no appendages in 12% of cases and the wild-type number of appendages in 48% of cases. (Klattenhoff et al., 2007) aub[QC42] flies show a disruption of the heterochromatic silencing of the P{T1} reporter in line P{T1}T190-177 and of the P{T1(-FRT)} reporter in line P{T1(-FRT)}T190-177. (Haynes et al., 2006) Perinuclear nuage appears smoother than normal in aub[HN]/aub[QC42] nurse cells, but nuage particles are unaffected. (Snee and Macdonald, 2004)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement Reference aub^HN/aub^QC42 has dorsal appendage phenotype, suppressible by lok^p6/lok^p6/lok^p6/lok^p6 (Klattenhoff et al., 2007) aub^HN/aub^QC42 has dorsal appendage phenotype, suppressible by mei-41^D3/mei-41^D3 (Klattenhoff et al., 2007)
Suppressor of
Statement Reference aub^QC42 is a suppressor of phenotype of osk^{bcd.3UTR.T:Zzzz\His6} (Wilson et al., 1996, Wilson et al., 1996)
Additional Comments
Genetic Interactions
Statement Reference The fused dorsal appendage phenotype of aub[HN]/aub[QC42] eggs is suppressed in lok[p6]; aub[HN]/aub[QC42] eggs with 98% of double mutants showing wild-type appendage morphology. The mei-41[D3] mutation suppresses the fused dorsal appendage phenotype of aub[HN]/aub[QC42] eggs; 85% of mei-41[D3]; aub[HN]/aub[QC42] eggs show wild-type appendage morphology. (Klattenhoff et al., 2007)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	4968 w¹¹¹⁸; aub^QC42 cn¹ bw¹/CyO, P{sevRas1.V12}FK1
Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	aub^QC42 (Klattenhoff et al., 2007, Ashraf et al., 2006, Grimaud et al., 2006, Vagin et al., 2006, Haynes et al., 2006, Snee and Macdonald, 2004, Usakin et al., 2007) aubergine^QC (Huynh et al., 2004) aubQC42 (Brennecke et al., 2007)
Name Synonym
Secondary FlyBase IDs

References ( 19 )
Research paper	Brennecke et al., 2007, Cell 128(6): 1089--1103 Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila. [FBrf0200494] Klattenhoff et al., 2007, Dev. Cell 12(1): 45--55 Drosophila rasiRNA pathway mutations disrupt embryonic axis specification through activation of an ATR/Chk2 DNA damage response. [FBrf0192439] Usakin et al., 2007, Genetics 176(2): 1343--1349 Transcription of the 1.688 satellite DNA family is under the control of RNA interference machinery in Drosophila melanogaster ovaries. [FBrf0201296] Grimaud et al., 2006, Cell 124(5): 957--971 RNAi components are required for nuclear clustering of Polycomb group response elements. [FBrf0189910] Haynes et al., 2006, Curr. Biol. 16(22): 2222--2227 Element 1360 and RNAi components contribute to HP1-dependent silencing of a pericentric reporter. [FBrf0193115] Savitsky et al., 2006, Genes Dev. 20(3): 345--354 Telomere elongation is under the control of the RNAi-based mechanism in the Drosophila germline. [FBrf0190561] Vagin et al., 2006, Science 313(5785): 320--324 A distinct small RNA pathway silences selfish genetic elements in the germline. [FBrf0194520] Cook et al., 2004, Cell 116(6): 817--829 The Drosophila SDE3 homolog armitage is required for oskar mRNA silencing and embryonic axis specification. [FBrf0174441] Huynh et al., 2004, Dev. Cell 6(5): 625--635 The Drosophila hnRNPA/B Homolog, Hrp48, Is Specifically Required for a Distinct Step in osk mRNA Localization. [FBrf0174543] Pal-Bhadra et al., 2004, Science 303(5658): 669--672 Heterochromatic silencing and HP1 localization in Drosophila are dependent on the RNAi machinery. [FBrf0168038] Snee and Macdonald, 2004, J. Cell Sci. 117(10): 2109--2120 Live imaging of nuage and polar granules: evidence against a precursor-product relationship and a novel role for Oskar in stabilization of polar granule components. [FBrf0174811] Findley et al., 2003, Development 130(5): 859--871 Maelstrom, a Drosophila spindle-class gene, encodes a protein that colocalizes with Vasa and RDE1/AGO1 homolog, Aubergine, in nuage. [FBrf0155724] Kennerdell et al., 2002, Genes Dev. 16(15): 1884--1889 RNAi is activated during Drosophila oocyte maturation in a manner dependent on aubergine and spindle-E. [FBrf0151326] Jankovics et al., 2001, Genetics 158(3): 1177--1188 An interaction type of genetic screen reveals a role of the Rab11 gene in oskar mRNA localization in the developing Drosophila melanogaster oocyte. [FBrf0137253] Wilson et al., 1996, Development 122(5): 1631--1639 aubergine enhances oskar translation in the Drosophila ovary. [FBrf0087782] Wilson et al., 1996, Dev. Genet. 19(3): 199--209 Novel genetic screen for genes involved in posterior body patterning in Drosophila. [FBrf0090859] Schupbach and Wieschaus, 1991, Genetics 129: 1119--1136 Female sterile mutations on the second chromosome of Drosophila melanogaster. [FBrf0054123]
Supplementary material	Ashraf et al., 2006, Cell 124(1): Supplemental Data. [FBrf0198682] Vagin et al., 2006, Science 313(5785): [FBrf0198717]

Allele Dmel\aubQC42

Allele Dmel\aub^QC42