Allele Dmel\Egfr^f5

General Information
Symbol	Dmel\Egfr^f5	Species	D. melanogaster
Name		FlyBase ID	FBal0003533
Feature type	allele	Created / Updated	2006-08-22/2006-08-22
Associated gene	Dmel\Egfr

Allele class	amorph, hypomorph
Mutagen	ethyl methanesulfonate
Nature of the Allele
Allele class	amorph (Schejter and Shilo, 1989) hypomorph (Clifford and Schupbach, 1989)
Mutagen	ethyl methanesulfonate (Tearle and Nusslein-Volhard, 1987, Clifford and Schupbach, 1989, Clifford and Schupbach, 1994)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Nucleotide substitution: G926A. Amino acid replacement: W186@. Stop codon is an amber. (Clifford and Schupbach, 1994)
Assay mode
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage \| recessive (Clifford and Schupbach, 1989, Schejter and Shilo, 1989, Clifford and Schupbach, 1994)
Phenotype Manifest In
	embryonic head epidermis (Dumstrei et al., 2002) embryonic optic lobe primordium (Dumstrei et al., 2002) embryonic head (Dumstrei et al., 2002) ventral nerve cord primordium (Dumstrei et al., 2002) optic lobe (Dumstrei et al., 1998) embryonic stomatogastric nervous system (De Velasco et al., 2004) embryonic corpus cardiacum (De Velasco et al., 2004)
Detailed Description
	Statement Reference severe allele Homozygotes and hemizygotes display an intermediate 'flb' phenotype. Embryos produced from heteroallelic combination with Egfr^t1 have a severe ventralised phenotype, reduction in size of their dorsal appendage. (Clifford and Schupbach, 1989) Enhances the female sterility and adult morphological defects of Egfr^t1. Rarely survives as transheterozygote with the semi-viable Egfr^top-CA allele. (Clifford and Schupbach, 1994) In mutant embryos, massive cell death occurs in the head. The visual system is almost entirely absent, though the Bolwig's organ remains. (Dumstrei et al., 1998) Egfr^f5 stage 11 embryos show domains of cell death in the anterior and posterior parts of the head. The posterior domain covers the optic placode. The apoptosis results in loss of major portions of the head epidermis, so that the brain is exposed in stage 14 embryos. The optic placode has almost disappeared by stage 14. The apoptosis is delayed in the optic placode relative to the apoptosis in the head epidermis. Cell death is also seen in the ventral neuroectoderm at stage 10/11. (Dumstrei et al., 2002) Late stage mutant embryos show a strong reduction in size of the corpus cardiacum compared to wild type and the stomatogastric nervous system is absent. (De Velasco et al., 2004)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement Reference Egfr^f5 has optic lobe phenotype, suppressible by Df(3L)H99 (Dumstrei et al., 1998)
NOT suppressed by
Statement Reference Egfr^f5 has phenotype, non-suppressible by eRF1[+]/eRF1^F2 (Chao et al., 2003)
Suppressor of
Statement Reference Egfr^f5 is a suppressor \| partially of eye phenotype of Krn^Scer\UAS.cRa, Scer\GAL4^GMR.PF (Reich and Shilo, 2002) Egfr^f5 is a suppressor \| partially of ommatidium phenotype of Krn^Scer\UAS.cRa, Scer\GAL4^GMR.PF (Reich and Shilo, 2002)
Other
Statement Reference Df(3L)H99, Egfr^f5 has Bolwig's organ primordium phenotype (Dumstrei et al., 2002) Df(3L)H99, Egfr^f5 has embryonic optic lobe primordium phenotype (Dumstrei et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference The optic lobes are restored in double mutants with Df(3L)H99. (Dumstrei et al., 1998) The optic placode fails to invaginate in Egfr^f5 ; Df(3L)H99 embryos, leaving it exposed at the surface of the embryo. Cells that would normally separate from the placode and become the Bolwig's organ remain part of the placode. These cells show a typical epithelial phenotype and are structurally indistinguishable from the surrounding optic lobe cells (although they express neuronal markers). In some cases, outgrowth of short, axon-like processes is seen. (Dumstrei et al., 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer
	Selected as: Embryonic lethal.
Comments
	Mutation of Egfr that coordinately affects all gene activities, a class I lesion. The allelic series for class I lesions: Egfr^t2 = Egfr^t1 < Egfr^top-EC20 < Egfr^f7 = Egfr^f1 = Egfr^flb-2E07 < Egfr^top-EE39 = Egfr^top-ED26 = Egfr^f5 < Egfr^f9 = Egfr^f10 = Egfr^f2 = Egfr^top-EE42 = Egfr^f11 = Egfr^f24 = Egfr^f3 = Df(2R)Egfr3. (Clifford and Schupbach, 1989) Double mutants for Egfr and sdt or crb show an enhancement of the sdt or crb mutant phenotype. (Tepass and Knust, 1993) Class I allele. (Clifford and Schupbach, 1994)
Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	Egfr^f5 flb flb^2G31 (De Velasco et al., 2004, Chang et al., 2003, Reich and Shilo, 2002, Raz et al., 1991, Schejter and Shilo, 1989, ) flb^IIG (Tearle and Nusslein-Volhard, 1987) top^2G31 (Clifford and Schupbach, 1994, Clifford and Schupbach, 1989)
Name Synonym	faint little ball
Secondary FlyBase IDs

References ( 14 )
Research paper	De Velasco et al., 2004, Dev. Biol. 274(2): 280--294 Embryonic development of the Drosophila corpus cardiacum, a neuroendocrine gland with similarity to the vertebrate pituitary, is controlled by sine oeulis and glass. [FBrf0179777] Chang et al., 2003, Dev. Biol. 263(1): 103--113 Antagonistic relationship between Dpp and EGFR signaling in Drosophila head patterning. [FBrf0173075] Chao et al., 2003, Genetics 165(2): 601--612 Mutations in eukaryotic release factors 1 and 3 act as general nonsense suppressors in Drosophila. [FBrf0167661] Dumstrei et al., 2002, Development 129(17): 3983--3994 Interaction between EGFR signaling and DE-cadherin during nervous system morphogenesis. [FBrf0151275] Reich and Shilo, 2002, EMBO J. 21(16): 4287--4296 Keren, a new ligand of the Drosophila epidermal growth factor receptor, undergoes two modes of cleavage. [FBrf0151286] Daniel et al., 1999, Development 126(13): 2945--2954 The control of cell fate in the embryonic visual system by atonal, tailless and EGFR signaling. [FBrf0108690] Dumstrei et al., 1998, Development 125(17): 3417--3426 EGFR signaling is required for the differentiation and maintenance of neural progenitors along the dorsal midline of the Drosophila embryonic head. [FBrf0104745] Clifford and Schupbach, 1994, Genetics 137(2): 531--550 Molecular analysis of the Drosophila EGF receptor homolog reveals that several genetically defined classes of alleles cluster in subdomains of the receptor protein. [FBrf0072848] Tepass and Knust, 1993, Dev. Biol. 159(1): 311--326 Crumbs and stardust act in a genetic pathway that controls the organization of epithelia in Drosophila melanogaster. [FBrf0058055] Raz et al., 1991, Genetics 129: 191--201 Interallelic complementation among DER/flb alleles: implications for the mechanism of signal transduction by receptor-tyrosine kinases. [FBrf0054136] Clifford and Schupbach, 1989, Genetics 123: 771--787 Coordinately and differentially mutable activities of torpedo, the Drosophila melanogaster homolog of the vertebrate EGF receptor gene. [FBrf0049928] Schejter and Shilo, 1989, Cell 56: 1093--1104 The Drosophila EGF receptor homolog (DER) gene is allelic to faint little ball, a locus essential for embryonic development. [FBrf0049332] Nusslein-Volhard et al., 1984, Roux Arch. dev. Biol. 193: 267--282 Mutations affecting the pattern of the larval cuticle in Drosophila melanogaster. [FBrf0041708]
Stock list	Tearle and Nusslein-Volhard, 1987, D. I. S. 66: 209--269 Tubingen mutants and stock list. [FBrf0045941]

Allele Dmel\Egfrf5

Allele Dmel\Egfr^f5