Aberration Dmel\Df(3R)gro^b32.2

General Information
Symbol	Dmel\Df(3R)gro^b32.2	Species	D. melanogaster
Name		FlyBase ID	FBab0022421
Feature type	chromosomal_deletion	Created / Updated	2006-08-22/2006-08-22

Formalized genetic data	boss << bk1 << HLHmδ << bk2 hits gro
Sequence coordinates
Deleted segment	96F9--96F11
Duplicated segment
Computed Breakpoints include	[96F9];[96F11]
Breakpoints Inherited
Nature of the Aberration
Cytological Order
Progenitor	gro^ry78 (Schrons et al., 1992)
Mutagen	Δ2-3 (Schrons et al., 1992)
Class of aberration (relative to progenitor)	chromosomal_deletion (Schrons et al., 1992)
Breakpoints
Causes alleles	gro^b32.2 (de Celis et al., 1996)
Carries alleles
Transposon Insertions
Genetic mapping information
Comments
Comments on Cytology
	Left limit of break 1 from non-inclusion of boss (FBrf0056226) Right limit of break 1 from inclusion of HLHmβ (FBrf0085207) Left limit of break 2 from inclusion of E(spl) (FBrf0056226) Right limit of break 2 from non-inclusion of gro (FBrf0056226)
Molecularly Mapped Breakpoints

Sequence Crossreferences
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name
Gene Deletion & Duplication Data
Genes Deleted / Disrupted
Complementation Data
Completely deleted / disrupted	HLHmδ (Heitzler et al., 1996, de Celis et al., 1996, de Celis et al., 1997, Ligoxygakis et al., 1998) HLHmγ (Heitzler et al., 1996, de Celis et al., 1996, de Celis et al., 1997, Ligoxygakis et al., 1998) HLHm3 (Heitzler et al., 1996, de Celis et al., 1996, de Celis et al., 1997, Ligoxygakis et al., 1998) HLHm7 (Heitzler et al., 1996, de Celis et al., 1996, de Celis et al., 1997, Ligoxygakis et al., 1998) E(spl) (Schrons et al., 1992, de Celis et al., 1996, Ligoxygakis et al., 1998) gro (Heitzler et al., 1996, de Celis et al., 1997) m4 (de Celis et al., 1996) HLHmβ (Heitzler et al., 1996, de Celis et al., 1996, de Celis et al., 1997, Ligoxygakis et al., 1998) HLHm5 (Heitzler et al., 1996, de Celis et al., 1996, de Celis et al., 1997, Ligoxygakis et al., 1998)
Partially deleted / disrupted	gro (Schrons et al., 1992, de Celis et al., 1996, Ligoxygakis et al., 1998)
Molecular Data
Genes NOT Deleted / Disrupted
Complementation Data
	boss (Schrons et al., 1992)
Molecular Data
Genes Duplicated
Complementation Data
Molecular Data
Genes NOT Duplicated
Complementation Data
Molecular Data
Related Comments
	Df(3R)gro^b32.2 does not delete gro but it is nevertheless affected. (Ligoxygakis et al., 1998)
Phenotypic Data
In combination with other aberrations
NOT in combination with other aberrations	Df(3R)gro^b32.2 gro^+t10.4 clones differentiate dense clumps of microchaetes that are frequently seen to be adjacent to one another. A small amount of epidermis differentiates and there are areas where bristles are intermingled with epidermal hairs. Small tufts of macrochaetes can be seen. (Heitzler et al., 1996) Df(3R)gro^b32.2 gro^+t10.4 clones in the eye disc show a cell autonomous neurogenic phenotype. (Ligoxygakis et al., 1998) Df(3R)gro^b32.2 follicle cells clones carrying gro^+t10.4 do not undergo extra cell divisions compared to wild type. (Deng et al., 2001) Scer\GAL4^da.G32-mediated coexpression of l(1)sc^Scer\UAS.cHa and da^Scer\UAS.cGa cause increase of cells in the CNS: brain lobes are enlarged (protruding through the holes in the cephalic epidermis), the ventral cord shows regional enlargement and sensory organs contain a large number of neurons. Neural hyperplasia is also increased, epidermis, fore- and hindgut, tracheal tree and salivary glands are completely neuralised. (Giebel et al., 1997) Complements the gro¹ allele. Clones in the thorax generate dense clusters of bristles at the locations that the macrochaetae develop. The microchaetae differentiate at high density forming large fields of bristles. The phenotype suggests that the E(spl) complex genes are required for segregation of the SOPs but not for their further differentiation. Duplicated trichogens occasionally occur in these clones, associated with fused tormogens, due to close packing of SOPs, rather than changes in SOP progeny identity. Clones in the wing that reach the wing margin show a loss of sensory structures along the wing margin. Wing veins also appear thicker. (de Celis et al., 1996) Expression of E(spl)^Scer\UAS.cNa under the influence of Scer\GAL4^da.G32 attenuates the mutant phenotype of Df(3R)gro^b32.2. A weaker attenuation is seen with HLHm5^Scer\UAS.cNa. Simultaneous expression of E(spl)^Scer\UAS.cNa or HLHm5^Scer\UAS.cNa with E(spl)^{1,bd-.Scer\UAS} driven by Scer\GAL4^da.G32 causes severe neural hypoplasia in embryos and adults, and reduces the level of neural hyperplasia in Df(3R)gro^b32.2. (Nakao and Campos-Ortega, 1996) Homozygous clones develop densely packed microchaetae within the clone. (Ramain et al., 2001) Homozygous clones in the leg form normal joints even when they span more than one segment and show differentiation of ectopic sensory organs. (de Celis et al., 1998) In a gro^+t9.2 background, Df(3R)gro^b32.2/+ or Df(3R)gro^b32.2/Df(3R)E(spl)-r72.1 strongly suppresses the bristle loss phenotype of H²⁰/H^E31. (Bang et al., 1995) Somatic clones in the follical cells, do not have any detectable phenotype. (Lopez-Schier and St. Johnston, 2001) Ventral mitotic clone in the wing causes thickening of the central component of wing vein LIII but does not affect vein differentiation in the opposite surface. Clones induced in a rho^ve-1 vn¹ mutant background that cover the vein LV territory do not differentiate a vein. (de Celis et al., 1997)
Position Effect Variegation Data

Stocks ( 0 )

Notes on Origin
Discoverer

Balancer / Genotype Variants of the Aberration

Separable Components

Other Comments

Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	Df(3R)(spl)^b32.2 (Koelzer and Klein, 2003) Df(3R)b^32.2 (Lecourtois and Schweisguth, 1998) Df(3R)E(spl)^b32.2 (Tapanes-Castillo and Baylies, 2004, Ramain et al., 2001) Df(3R)E(spl)^grob32.2 (Cooper and Bray, 1999, de Celis et al., 1998) Df(3R)E(spl)^R-b32.2 (Giebel et al., 1997) Df(3R)gro^b32.2 Df(3R)gro^ry78R-b32.2 (Schrons et al., 1992) E(spl)^b32.2 (Venken, 2006, Apidianakis et al., 1999, de Celis et al., 1997, de Celis et al., 1996, Bang et al., 1995) E(spl)<up>b32.2 gro+</up> (Pi et al., 2001) gro^b32.2 (Lopez-Schier and St. Johnston, 2001, )
Name Synonym
Secondary FlyBase IDs

References ( 25 )

Generate a list of	All references relating to this aberration ( 25 )

List References by type	Research paper ( 23 ) Supplementary material ( 1 ) FlyBase analysis ( 1 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2006 Show research papers ...

Aberration Dmel\Df(3R)grob32.2

Aberration Dmel\Df(3R)gro^b32.2